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Inflammatory bowel diseases (IBD) is a type of disorders characterized by chronic inflammation in gastrointestinal tract. The incidence and prevalence of IBD have been increasing over the last 50 years. Intestinal surgery and medication of steroids or anti-TNFα drug have been extensively used with undesirable side effects. Modulation of gut immune responses through hostmicrobe interaction is a new therapeutic approach to cure IBD without adverse effects. The deficiency of Faecalibacterium prausnitzii has been proven to play a role in pathology of Crohn’s disease (CD). Recently, the anti-inflammatory properties of a F.prausnitzii’s protein called Microbial Anti-inflammatory Molecule (MAM) have been published. In in vitro studies, MAM showed anti-inflammatory activity by blocking NF-κB signaling pathway. In vivo studies indicated that MAM allowed faster recovery in chemically-induced colitis model in mice. Because F.prausnitzii is a strictly anaerobic bacterium, therefore, to produce a considerable amount of MAM, it is necessary to construct a plasmid and insert to other cultivable bacteria strains. Different bacteria strains were tested as Escherichia coli or Lactococcus lactis, however, MAM produced was always insoluble. Thus, we made a new MAM plasmid construction in Saccharomyces cerevisiae, in which we successfully produce soluble and active MAM. However, purification of MAM from the whole yeast proteins, as well as to increase the production yield is the challenges and still ongoing steps of the project.
I obtained a Master’s degree in Food Science and Technology at Kasetsart University, Thailand, under a joint program with University of British Columbia, Canada. After working as a research assistant for one year, I pursued a PhD program at Wageningen University, The Netherlands, with the focus on the discipline “immune modulation by food”. This allowed me to further develop my skills and techniques in Food chemistry and also in the field of Immunology. After obtaining my doctor’s degree, I received a permanent position to become a lecturer/researcher at Kasetsart University in the department of Food Science and Technology to Thailand since January 2013. Over the past years, I have been granted several projects as both a Principal Investigator (PI) and a co-researcher from Thai granting agencies. During the AgreenSkills mobility program in the Probihôte at MICALIS Institute, INRA, I have gained practical experiences in gut immune modulation, and get to know researchers in the group. The first PhD student from my faculty in Thailand will be coming to spend 6-8 months period under the Probihote group. This is the start for institute collaboration and I believe, more to come.
Chanput W., Krueyos N., Ritthiruangdej P., 2016. Antioxidative assays as markers for anti-inflammatory activity of flavonoids. International Immunopharmacology. 40: 170-175.
Chanput W., Mes J.J. and Wichers H.J., 2014. THP-1 cell line: an in vitro model for immune modulation approach. Review. International Immunopharmacology. 23(1):37-45.
Bastiaan-Net S.*, Chanput W.*, Hertz A., Zwittink R.D. Mes J.J. and Wichers H.J., 2013. Biochemical and functional characterization of recombinant fungal immunomodulatory proteins (rFIPs). 2013. International Immunopharmacology. 15: 167-175. * These authors contributed equally to this work
Chanput W., Reitsma M., Kleinjans L., Mes J.J., Savelkoul H.F.J. and Wichers H.J., 2012. β-glucans are involved in immune-modulation of THP-1 macrophages. Molecular Nutrition and Food Research. 56: 822-833.
Chanput W., Mes J.J., Vreeburg R.A.M., Savelkoul H.F.J. and Wichers H.J., 2010. Transcription profiles of LPSstimulated THP-1 monocytes and macrophages: A tool to study inflammation modulating effects of food derived compounds. Food and Function. 1: 254-261. Doi: 10.1039/C0FO00113A.