FranceCountry of destination:
United States of America
The precise and coordinated regulation of gene expression, as well as its epigenetic maintenance during cell divisions, is central to the development of the organism and its response to stimuli from the environment. Recently, RNA Polymerase II (PolII) pausing has been shown to play important roles in gene expression, RNA processing and chromatin organization in animals. Evidence that PolII pausing also occurs in plants is still lacking. Based on encouraging preliminary results from Scott Michaels' group, we will evaluate if PolII pausing occurs in Arabidopsis thaliana and how it could interact with transcriptional and epigenetic mechanisms using genome-wide approaches and bioinformatics.
After obtaining my diploma of engineering in agronomy and my MSc at Agrocampus Ouest (2000), I obtained my PhD, on transcriptional regulation by nuclear receptors (PPARalpha and RXR, supervisor: T. Pineau) in 2007. I was appointed research engineer at INRA during my PhD (2004) and I co-directed the group of Integrative Toxicology & Metabolism for 4 years (20072011). I next created a transcriptomic platform at INRA ToxAlim (2011-2013). During these years, I led significant research projects that contributed to the development of ToxAlim. In 2013-2015, I joined the group of Olivier Cuvier, where I studied chromatin organization and transcription in Drosophila cells using various genome-wide approaches based on NGS. Thanks to the AgreenSkills+ program, I then joined the group of Scott Michaels at Indiana University (2015-2017) where we studied a new family of transcription elongation factors and deciphered their role on RNA polymerase II processivity, chromatin organization and gene expression. Throughout my career, I have balanced biology with bioinformatics and biostatistics and gained experience in the production and analysis of different types of microarray and NGS data.
Stadelmayer B, Micas G, Gamot A, Martin PG, Malirat N, et al., 2014. Integrator complex regulates NELF-mediated RNA Polymerase II pause/release and processivity at coding genes. Nature Communications. 5:5531. Doi: 10.1038/ncomms6531.
Liang J, Lacroix L, Gamot A, Cuddapah S, Queille S, Lhoumaud P, Lepetit P, Martin PG, et al., 2014. Chromatin immunoprecipitation indirect peaks highlight long-range interactions of insulator proteins and Pol II pausing. Mol Cell. 53(4):672-81.
Labialle S, Marty V, Bortolin-Cavaillé ML, HoareauOsman M, Pradère JP, Valet P, Martin PG, Cavaillé J., 2014. The miR-379/miR-410 cluster at the imprinted Dlk1-Dio3 domain controls neonatal metabolic adaptation. EMBO J. 33(19):2216-30.
Roques BB, Leghait J, Lacroix MZ, Lasserre F, Pineau T, Viguié C, Martin PG, 2013. The nuclear receptors pregnane X receptor and constitutive androstane receptor contribute to the impact of fipronil on hepatic gene expression linked to thyroid hormone metabolism. Biochem Pharmacol. 86(7):997-1039.
Marmugi A, Ducheix S, Lasserre F, Polizzi A, Paris A, Priymenko N, Bertrand-Michel J, Pineau T, Guillou H, Martin PG, Mselli-Lakhal L., 2012. Low doses of bisphenol A induce gene expression related to lipid synthesis and trigger triglyceride accumulation in adult mouse liver. Hepatology. 55(2):395-407.
Agreenskills + fellowship (2015-2017). Project in the laboratory of Scott D Michaels at Indiana University. IN, USA.
PhD scorlarship from the Ministère de l'éducation nationale, de l'enseignement supérieur et de la recherché (2000-2003) and 1 year extension (2004) from the Ligue Contre le Cancer (only 3 months used due to my recruitment at INRA in Jan 2004)