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Mohammed Akli Ayoub

Mohammed Akli Ayoub


Agreenskills+
session, year:
2014 1st

Status:
Former fellow

Receiving laboratory:
PRC Reproductive and Behavioural Physiology, Tours

Country of origin:

Saudi Arabia

Country of destination:

France

Last available contact

Email(s):
mohammed.ayoub@inra.fr
ayoubmohamed@yahoo.fr
Website(s):
https://intranet6.val-de-loire.i...

CV:
Download Curriculum Vitae

Mobility project

Identification and Pharmacological Profiling of Selective Nanobodies Targeting G Protein-Coupled Receptors – Towards in vivo Applications

My project aims to develop new generation of biologicals targeting GPCRs. These biologicals are antibody fragments generated from camelids, called nanobodies. I am in charge of developing a battery of molecular, biochemical, and signalling assays that will be instrumental for the development of the nanobodies. Indeed, nanobodies displaying distinct activation profiles are expected: activators versus inhibitors, full versus partial activities, competitors versus allosteric binders,… Each profile could potentially lead to valuable in vivo action; therefore accurate screening and characterization will be carried out. Furthermore, the potential clinical and agronomic applications of the nanobodies will be assessed using in vivo models. 

Biography & research interests

I was born in Algeria and did my undergraduate and postgraduate studies in Biochemistry at the University of Paris XI. I obtained my PhD in 2003 from University Paris XI having carried out my research project at Cochin Institute (Paris). Then, I joined the Functional Genomics Institute in Montpellier for my first postdoctoral fellow from 2004 to 2009. In August 2009, I moved to Perth (Australia) where I was employed as full-time Research Assistant Professor by the University of Western Australia to work at Harry Perkins Institute of Medical Research. From 2012 to 2014, I have been appointed as Assistant Professor in the Biochemistry department in King Saud University (Saudi Arabia). In July 2014, I returned back to France as LE STUDIUM Loire Valley Institute for Advanced Studies and Marie-Curie/ AgreenSkills fellow to work at INRA-Val de Loire (Tours, France). Since January 2017, I am appointed as Assistant Professor in College of Science, Biology Department at United Arab Emirates University in Al Ain (UAE). My scientific career has two major focus: the biology of cell surface receptors and technology development based on BRET and FRET approaches applied to study different aspects of receptor function. During my career, I have published more than 50 publications with an average impact factor of 6 and having received in total more than 2500 citations.

Selected publications

Reiter E, Ayoub MA, Pellissier L.P, Landomiel F, Musnier A, Trefier A, Gandia J, De Pascali F, Tahir S, Yvinec R, Bruneau G, Poupon A, and Crepieux P. 2017. B-arrestin signalling and bias in hormone-responsive GPCRs. Mol Cell Endocrinol., 449:28-41.

Ayoub MA, Crepieux P, Koglin M, Parmentier M, Pin JP, Poupon A, Reiter E, Smit M, Steyaert J, Watier H, and Wilkinson T. 2017. Antibodies targeting G protein-coupled receptors: Recent advances and therapeutic challenges. MAbs. 9(5):735-741.

Riccetti L, Yvinec R, Klett D, Gallay N, Combarnous Y, Reiter E, Simoni M, Casarini L, and Ayoub MA, 2017.Human Luteinizing Hormone and Chorionic Gonadotropin Display Biased Agonism at the LH and LH/CG. Receptors Scientific Reports. 7(1):940. Doi: 10.1038/s41598-017-01078-8.

Sposini S, Jean-Alphonse FG, Ayoub MA, Oqua A, Lavery S, Brosens JJ, Reiter E, and Hanyaloglu AC., 2017. Integration of GPCR Signaling and Sorting from Very Early Endosomes via Opposing APPL1 Mechanisms. Cell Reports, 21(10): 2855-2867.

Ayoub MA, Yvinec R, Jegot G, Dias JA, Poli S.M, Poupon A, Crepieux P, and Reiter E., 2016. Profiling of FSHR negative allosteric modulators on LH/CGR reveals biased antagonism with implications in steroidogenesis. Mol Cell Endocrinol. 436:10-22.

 

Awards & patents

Travel Award of GDR GPCR-DiscoveRx - DiscoveRx European GPCR Technology Symposuim. Elucidating GPCR Functional Selectivity and Biased Agonism: Novel Opportunities for Drug Development. 12 May 2015 - Cambridge, UK.

AgreenSkills+ Fellowship - 1st Selection round 2014 (funding duration: 36 months).

The International Meeting Travel Grant of King Saud University to attend The Gordon Conference - Molecular Pharmacology. 28 April - 03 May 2013 - II Ciocco, Lucca, Italy.

The Supplementary Travel Grant of the University of Western Australia to support my participation to The VIth Annual Meeting of the Molecular Pharmacology of G Protein-Coupled Receptors: 2-4 December 2010 - Melbourne, Australia.

Travel Award of The Gordon Conference – Molecular Pharmacology: 31 May- 5 June 2009 - II Ciocco, Lucca, Italy.

The XIth European Pineal and Biological Rhythms Society Symposium: 18-23 July 2002 - Aberdeen, Scotland.

Naturalia & Biologia travel award to attend the XXXIVth Congress of the International Union of Physiological Sciences. 21-23 August 2001 - Adelaide, Australia.

Talks in annual meetings

Modulating GPCRs’ Biological Activities with Nanobodies
2015 / P1 Molecular and Cellular / by Mohammed Akli Ayoub / Fellows Speed Presentation

Keywords

GPCRs, nanobodies, biased ligands, G proteins, arrestins, BRET, FRET, TR-FRET, signalling