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The objective of my AgreenSkills postdoctoral project was to develop an in vitro callus assay to recreate a ‘callus graft’ interface in tissue culture and in vitro micro-grafting in grapevine. Grafting is widely used in the agriculture of fruit crops but it is poorly understand yet. We proposed to study the role of plasmodesmata connections between the scion and rootstock in grafted plant development. This project was part of a larger project of the receiving laboratory, GRAFTACTIONS, which is concerned with the gene expression, protein accumulation and structural developments at the graft interface in different scion/rootstock combinations of grapevine.
I started my PhD studies in Plant Biotechnology in Biotechnology of fruit tree Group (Plant Breeding Department) in the CEBASCSIC Research Center, under supervision of Dr. Jose Antonio Hernández Cortés. During my PhD, my work was focused in plant response to biotic stress caused by Plum pox virus infection at antioxidant systems level, especially in woody plants peach. I defended my PhD Thesis in July 2011. After my PhD, thanks to AgreenSkills program, I had the opportunity to work in a very innovative and ambitious project as a post-doctoral researcher in Ecophysiologie et Génomique Fonctionnelle de la Vigne group, in INRA Bordeaux. This project was focused on the study of plasmodesmata connectivity at the graft interface and on the keys steps in the grafting process. Since 2015 I’m working at the University of the Balearic Islands as post-doc researcher, focusing my research in the eco-physiological and biochemical responses to stress conditions, specially drought and cold tolerance. Currently I am involved in others several ecophysiological projects and I collaborate with several researcher groups in University of Concepcion (Chile) and University of Wollongong (Australia) thanks to two fellowships granted for short stays in 2016, Santander Universities Fellowships for Young Researchers and Endeavour fellowships respectively. Moreover, I have participated in one Antarctic Spanish campaigns and one Artic expedition during 2017.
*Hernandez JA, Diaz-Vivancos P. Barba-Espin G. Clemente-Moreno MJ, 2017. On the role of salicylic acid in plant responses to environmental stresses. In: Salicylic Acid: A Multifaceted Hormone. R. Nazar et al. (eds.), Springer Nature ISBN: 978-981-10-6067-0
Néstor Fernández Del Saz, Igor Florez-Sarasa, María José Clemente-Moreno, Haytem Mhadhbi, Jaume Flexas, Alisdair Fernie, Miquel Ribas-Carbó, 2016. Salinity tolerance is related to cyanide-resistant alternative respiration in Medicago truncatula. Plant, Cell & Environment, 39, 23612369.
*Clemente-Moreno MJ, Hernandez J.A., Diaz-Vivancos P. 2014. Sharka: How plants respond to Plum pox virus infection? J. Exp. Bot. 66(1):25-35.
*Clemente-Moreno MJ, Hevin C, Ollat N, Cookson SJ, 2014. Developments at the graft interface in homo- and heterografts: Gene expression and histological changes during the first month after grafting. Plant Signaling & Behavior. 25; 9. Doi:10.4161/psb.28852 / e28852.
*Clemente-Moreno MJ, Diaz-Viviancos P, Rubio M, Fernandez-Garcia N, Hernandez JA, 2013. Chloroplast protection in PPV-infected peach plants by OTC treatments: Effect in the proteome. Plant Cell and Environm 36: 640654.
Patent entitled: “Treatments to increase vigour in fruit trees under in vitro conditions” (submitted 2011). Ref: P201130888.