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The clock mechanisms have been described as a complex network of multiple interconnected transcriptional/translational feedback loops controlling a wide variety of vital processes on organism, tissue, cellular and molecular levels. New evidences indicate a non-transcriptional timing, well known in prokaryotes, is also present in eukaryotes. The aims are to characterize for the first time in eukaryotes the ancestral non-transcriptional clock and to determine how protein phosphorylation affects the clock system. The mechanisms will be described firstly using a reduced model system, the pico-alga Ostreococcus tauri, and then confirmed in model plant Arabidopsis thaliana.
I studied Biology Science at the University of Montpellier where I obtained a Master in Plant Functional Biology then a doctoral fellowship from INRA (Contrat Jeune Scientifique) in 2010. My PhD was carried out at INRA of Montpellier under the supervision of Dr Christophe Maurel and was aimed to contribute to determine the genetic, molecular and cellular bases of water transport regulation by water channel aquaporins in leaves of Arabidopsis thaliana in response to light. I joined the lab of Professor Andrew Millar at the University of Edinburgh (UK) as a post-doctoral research associate thanks to an AgreenSkills fellowship. I studied the contribution of non-transcriptional mechanisms to biological timekeeping of a new model organism for plant Systems Biology the pico-alga Ostreococcus tauri. Currently, I am elucidating how the phytochrome light receptors regulate chloroplast protein abundance and activity in the lab of Professor Karen Halliday at the University of Edinburgh. Through my projects, I enriched my knowledge of the photobiology, the posttranscriptional modifications, and I learnt the fundamentals of Systems Biology for which the interest growths along with the increasing of bioinformatics data.
Corratgé-Faillie C, Ronzier E, Sanchez F, Prado K, Kim JH, Lanciano S, Leonhardt N, Lacombe B, Xiong TC., 2017. The Arabidopsis guard cell outward potassium channel GORK is regulated by CPK33. FEBS Lett. 591(13):1982-1992.
Ronzier E, Corratgé-Faillie C, Sanchez F, Prado K, Brière C, Leonhardt N, Thibaud JB, Xiong TC., 2014. CPK13, a non-canonical Ca2+-dependent protein kinase, specifically inhibits KAT2 and KAT1 Shaker K+ channels and reduces stomatal opening. Plant Physiol 166(1):314- 326.
di Pietro M, Vialaret J, Li G, Hem S, Prado K, Rossignol M, Maurel C, Santoni V., 2013. Coordinated post- translational responses of aquaporins to abiotic and nutritional stimuli in Arabidopsis roots. Mol Cell Proteomics, 12:3886-3897.
Prado K, Maurel C., 2013. Regulation of leaf hydraulics: from molecular to whole plant levels. Front. Plant Sci. 4: 255.
Prado K, Boursiac Y, Tournaire-Roux C, Monneuse JM, Postaire O, Da Ines O, Schaffner AR, Hem S, Santoni V, Maurel C., 2013. Regulation of Arabidopsis leaf hydraulics involves light-dependent phosphorylation of aquaporins in veins. Plant Cell 25: 1029-1039.
INRA CJS contract, Agreenskills, 2nd session, 2013