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The xylem plays a central role in the long-distance transport of water within the plant. Failure in hydraulic function due to vascular diseases and water deficit limit productivity or induce mortality across a wide range of plant systems. The cultivation of grapevine, Vitis vinifera L. sativa, has a meaningful place in the French economy. To date, the sustainability of this industry is threaten by the progression of vascular diseases and climate change. Among trunk pathologies affecting grapevine, the Esca disease is of the most destructive. Recent efforts led to the identification of a morphological trait of the xylem that correlates with the degree of resistance of grapevine cultivars to one of its fungal causal agent, Phaeomoniella chlamydospora. The xylem morphology is also known to impact on the ability of plants to maintain hydraulic function and survive under water shortage. The structure of the xylem is genotype-dependent but also presents a developmental plasticity that responds to environmental factors, such as the water availability. The xylem morphology represents a crossroad for the integration of various environmental stresses, and its study provides an enticing avenue to better understand how responses to abiotic and biotic stresses interact in perennial agro-systems and potentially converge. The PlastiXyl project objectives are (i) to better characterize the genetics and physiologic bases responsible for changes in xylem morphology by using F1 and F2 grapevine progenies from an interspecific cross (V. vinifera x V. riparia), and (ii) to determine its consequences for xylem susceptibility to P. chlamydospora and drought stress-induced embolism. This interdisciplinary project collaboration with groups of researchers with recognized expertise in grapevine genetics, xylem physiology and pathology. This innovative multidisciplinary approach will discover novel insights on some of the biggest issues the grapevine industry has to face in the EU, and improve ER's possibilities to reach a research position.
I grew up in Bordeaux, France, where I graduated with a Master in Plant Biology and Biotechnology. I graduated with a PhD in Plant-Microbe Interactions at the National Polytechnics Institute of Toulouse, France, in 2012. My thesis works was dealing with the development of quantitative molecular methods for the detection of the main vascular fungal pathogens in grapevine. I also addressed some aspects related to the spatial organization of defense response into the grapevine wood. I moved into the USA right after I defended my PhD to work on a five-year funded project at the University of California Riverside. The goal of the project was to identified anatomical and biochemical markers of the resistance toward fungal vascular pathogens in grapevine and almond. Since Junuary 2018, I am conducted research at the INRA Bordeaux-Aquitaine. My project deals with the understanding of genetic and physiologic basis responsible for the morphological plasticity of the xylem in grapevine, and its effect on the resistance to vascular disease and drought.
Pouzoulet, J., Scudiero, E., Schiavon, M., & Rolshausen, P. E., 2017. Xylem vessel diameter affects the compartmentalization of the vascular pathogen Phaeomoniella chlamydospora in grapevine. Frontiers in plant science, 8, 1442. Doi: 10.3389/fpls.2017.01442.
Pouzoulet, J., Rolshausen, P.E., Schiavon, M., Bol, S., Travadon, R., Lawrence, D.P., Baumgartner, K., Comont, G., Corio-Costet, M.F., Pierron, R.J. and Besson, X., Jacques, A., 2017. A method to detect and quantify Eutypa lata and Diplodia seriata-complex DNA in grapevine pruning wounds. Plant Disease, 101 (8), 14701480.
Pierron RJG, Pouzoulet J, Couderc C, Judic E, Compant S and Jacques A., 2016. Variations in Early Response of Grapevine Wood Depending on Wound and Inoculation Combinations with Phaeoacremonium aleophilum and Phaeomoniella chlamydospora. Frontiers in plant science. 7:268. Doi: 10.3389/fpls.2016.00268.
Doll D. A., Rolshausen P. E., Pouzoulet, J., et al., 2015. First Report of Dothiorella iberica Causing Trunk and Scaffold Cankers of Almond in California. Plant disease, 99(8), 1185-1185.
Pouzoulet, J., Pivovaroff, A. L., Santiago, L. S., & Rolshausen, P. E., 2014. Can vessel dimension explain tolerance toward fungal vascular wilt diseases in woody plants? Lessons from Dutch elm disease and esca disease in grapevine. Frontiers in plant science, 5, 253. Doi: 10.3389/fpls.2014.00253.