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Dušan Velickovic

Dušan Velickovic

session, year:
2012 1st

Former fellow

Receiving laboratory:
UR BIA, Biopolymers, Interactions and Assemblies, Nantes

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Download Curriculum Vitae

Mobility project

In situ observation of polysaccharides in plant tissues by MALDI mass spectrometry

The objective of this mobility project is to implement the Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI MSI) method in order to observe the spatial distribution of cell wall polysaccharides in wheat grains. This technique has recently emerged as a powerful method for resolving both the spatial distribution and structures of many kinds of molecules in intact tissue sections. However, none of these MSI studies have focused on the imaging of complex cell-wall polysaccharides, despite the asymmetric distribution of these compounds throughout plant tissues and the important roles of these molecules regarding cell wall properties, plant tissue cohesion, organ development and defence processes. The two main components of wheat cell wall are arabinoxylans (AX) and mixed ?-glucans (MLG). Because their structural variability impacts the development and processing quality of wheat, obtaining adequate insight into the occurrence and spatial distribution of these biopolymers across the grain is of considerable interest. The approach applied in the mobility project involves the in situ enzymatic hydrolysis of cell wall polysaccharides, followed by analysis of the spatial distribution of the generated oligosaccharides using MALDI mass spectrometry.

Biography & research interests

I was born in December 1985 in Belgrade, Serbia. In 2008 I graduated at the Faculty of Chemistry of the University of Belgrade with average grade 9.47/10. In 2011, I defended my PhD at the University of Belgrade’s Faculty of Chemistry as the youngest PhD of the Department so far. From 20092010, I was employed as a Graduate researcher, from 2010-2012 as Associate researcher and from 2012 as Scientific Associate at the Faculty of Chemistry, University of Belgrade. From 2009-2011, I was hired as the assistant for the course “Chemistry of the Natural Products” at the Faculty of Chemistry University of Belgrade. In period 20122014 I was employed as post-doctoral researcher in mass spectrometry laboratory at INRA, Nantes. Since 2016 I am hired as post-doctoral researcher scientist in Mass Spec/ Magnetic Resonance group at Pacific Northwest National Laboratory (PNNL), Richland WA. My general research interests are MALDI and MS imaging; Ion mobility spectrometry; carbohydrate MS analysis; applications of enzymes in aqueous and non-aqueous systems and enzymatic kinetic.

Selected publications

Velickovic D., Chu, RK; Carrell, AA; Thomas, M; Pasa-Tolic, L; Weston, DJ; Anderton, CR, 2018. Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization. Analytical Chemistry, 90 (1), 702-707.

Ivic, JT; Milosavic, N; Dimitrijevic, A; Jankulovic, MG; Bezbradica, D; Kolarski, D; Velickovic, D, 2017. Synthesis of medium-chain length capsinoids from coconut oil catalyzed by Candida rugosa lipases. Food Chemistry, 218, 505-508.

Velickovic D., Saulnier L., Lhomme M., Damond A., Guillon F., Rogniaux H., 2016. Mass Spectrometric Imaging of Wheat (Triticum spp.) and Barley (Hordeum vulgare L.) Cultivars: Distribution of Major Cell Wall Polysaccharides According to Their Main Structural Features. Journal of Agricultural and Food Chemistry, 64 (32): 6249-6256.

Velickovic D., Herdier H., Philippe G., Marion D., Rogniaux H., Bakan B., 2014. Matrix-assisted laser desorption/ionization mass spectrometry imaging: a powerful tool for probing the molecular topology of plant cutin polymer. Plant Journal, 80 (5): 926-935.

Velickovic D., Ropartz D., Guillon F., Saulnier L., Rogniaux H., 2014. New insights into the structural and spatial variability of cell wall polysaccharides during wheat grain development, as revealed through MALDI mass spectrometry imaging. Journal of Experimental Botany, 65, 2079-2091.

Velickovic D., Dimitrijevi?, A., Bihelovi?, F., Bezbradica D., Jankov R., Milosavi? N., 2011. A highly efficient diastereoselective synthesis of ∝-isosalicin by maltase from Saccharomyces cerevisiae. Process Biochemistry, 46:16981702.

Awards & patents

2009.  Holder of a special recognition of the Serbian Chemical Society for outstanding achievement in the academic studies.

2008. Scholarship Fund for Young Talents of the Ministry of Youth and Sport of the Republic of Serbia.

2007. Scholarship from the Ministry of Education and Sports of the Republic of Serbia.

Talks in annual meetings

MALDI imaging of polysaccharides
2013 / P5 Products and Technology / by Dušan Velickovic / Fellows Speed Presentation


MALDI, imaging, polysaccharides, cell wall, plant