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Endosymbiotic bacteria of eukaryotes provide an expanded metabolic repertoire which confers an evolutionary advantage to their hosts when facing environmental challenges. As many insects, aphids keep an obligate nutritional symbiont, Buchnera, which provides essential amino acids and vitamins, enabling the aphid to feed on the nutritionally-unbalanced plant phloem. In the Lachninae subfamily, the loss of a symbiotic function in the Buchnera genome has triggered the establishment of a coobligate secondary endosymbiont. Within this subfamily, the genus Cinara is the most diverse and has managed to expand throughout the Holoartic by colonising a large set of conifer species. In addition, most Cinara species harbour the endosymbiont Serratia symbiotica, however, different Serratia strains present contrasting tissue tropisms in different hosts and vary greatly in their genomic content and morphological traits. Furthermore, in some lineages of Cinara, Serratia has been replaced by other bacterial lineages. The aim of this project is to understand the role of these endosymbiotic bacterial partners in the diversification of their hosts. It is expected that the similarities and differences among the symbionts' metabolic capabilities and aphid niches (plant species, feeding sites, and climatic niches) will elucidate the symbionts role in the diversification of their hosts. Through whole-genome sequencing and annotation of the different endosymbionts from Cinara species, representing about half of the genus species diversity and the range of ecological niches occupied by the genus, we aim to both infer the metabolic contributions of the symbionts and their phylogenetic relationships. Cospeciation analyses will be conducted, in order to infer the history of the association of the symbionts with Cinara. Using comparative genomics, in combination with phylogenetic methods, we will evaluate the contribution of Cinara' endosymbiotic consortia to the diversification of their hosts.
From 2006 to 2010, I studied my undergraduate in Genomic Sciences in Mexico at the National Autonomous University of Mexico (UNAM). During my last year, I performed research on the genomics and evolution of Serratia symbiotica, the secondary mutualistic co-obligate endosymbiotic bacterium of the the aphid Cinara tujafilina. During this period, I developed a great interest in both the genomic changes and metabolic adaptations that mutualistic symbiotic bacteria undergo during their Life cycle. Next, I moved to Spain to the Cavanilles Institute for Biodiversity and Evolutionary Biology (ICBiBE, Valencia), where I got my master’s degree in Molecular and Cell Biology and Genetics. Afterwards, I got a Marie Curie ITN Doctoral Fellowship (SYMBIOMICS) to do my PhD at the ICBiBE working on the comparative genomics of mutualistic co-obligate di-symbiotic systems in the Lachninae subfamily of aphids. During this period, I investigated the evolution of co-obligate symbiosis, symbiont replacement, and the metabolic bases of symbiont cooperation for the production of essential compounds for their hosts.
Meseguer A S, Manzano-Marín A, Coeur d’acier A, Clamens A-L, Godefroid M, Jousselin M., 2017. Buchnera has changed flatmate but the repeated replacement of co-obligate symbionts is not associated with the ecological expansions of their aphid hosts. Molecular Ecology, 26(8), 2363-2378.
Manzano-Marín, A., Szabó, G., Simon, J.-C., Horn, M., & Latorre, A., 2017. Happens in the best of subfamilies: establishment and repeated replacements of co-obligate secondary endosymbionts within Lachninae aphids. Environmental Microbiology, 19(1), 393-408.
Klein, A., Schrader, L., Gil, R., Manzano-Marín, A., Flórez, L., Wheeler, D., Oettler, J., 2016. A novel intracellular mutualistic bacterium in the invasive ant Cardiocondyla obscurior. The ISME Journal, 10(2), 376-388.
Manzano-Marín, A. & Latorre, A., 2016. Snapshots of a shrinking partner: Genome reduction in Serratia symbiotica. Scientific Reports, 6, 32590. Doi:10.1038/ srep32590.
Manzano-Marín, A., Simon, J.-C., & Latorre, A., 2016. Reinventing the Wheel and Making It Round Again: Evolutionary Convergence in Buchnera - Serratia Symbiotic Consortia between the Distantly Related Lachninae Aphids Tuberolachnus salignus and Cinara cedri. Genome Biology and Evolution, 8(5), 1440-1458.