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Gloria Torres-Cortés

Research Institute of Horticulture and Seeds, Inra, Agrocampus Ouest, University of Angers, Angers, France

Copiotrophic traits drive the assembly of bacterial microbial community during germination and emergence (scientific report)



Annual meeting: 2019

Fields-Topics: P3 Population and Ecosystems,P6 Applied Maths

Type of talk: Fellows Speed Presentation

Copiotrophic traits drive the assembly of bacterial microbial community during germination and emergence (scientific report)

Biography

I graduated in Biology at the University of Granada (Spain). My attraction for microbial diversity started while I was an undergraduate student and continued during my PhD at the “Estacion Experimental del Zaidin” (CSIC-Granada; Spain). During my PhD, I analyzed the bacterial rhizosphere community associated to different cactus in a natural park in Mexico. From a more applied perspective, I also studied the lipolytic enzymes and antibiotic resistance reservoir of these bacterial communities. After successfully obtaining my PhD, I decided to pursue the goal of completing the knowledge acquired during my PhD with some insight into fungal diversity. For this purpose, I joined the Genetic section of the University of Munich (LMU), where I would be working with arbuscular mycorrhizal fungi (AMF). During my first postdoctoral year I worked on the molecular characterization of AMF from environmental communities and during the last years, I was involved in the development and execution of a project focused on a type of Mycoplasma-related endobacteria of AMF. In this project, we provided evidence of trans-kingdom gene transfer between the endobacteria and their AMF host. Currently, I continue developing my scientific career in bacteria-fungal interactions and microbial diversity as an AgreenSkills fellow, studying bacterial and fungal communities associated with plant seeds.

Abstract

Copiotrophic traits drive the assembly of bacterial microbial community during germination and emergence (scientific report)

Microbial interactions occurring on and within seeds are key for plant fitness since seed associated microorganisms are the primary source of inoculum for the plant. The aim of this study is to uncover microbial traits implicated in the assembly of the seed microbiome during germination and plant emergence. With this purpose, we performed shotgun sequencing of microbial DNA and RNA from seeds, germinating seeds and seedlings of two plant species: bean and radish. We observed an enrichment ofEnterobacterialesandPseudomonadalesduring emergence and a group of functional traits linked to copiotrophy that could be responsible for this selection due to the increase of nutrient availability after germination. Moreover, representative bacterial isolates of taxa that are selected in seedlings showed faster bacterial growth rate in seed exudates media in comparison to seed-associated bacteria isolates. In addition, multifunctional redundancy analyses showed that radish-associated microbial assemblages are more resistant to invasion by alien species at the seedling stage than bean microbiota. Finally, binning of metagenomics contigs results in the reconstruction of population genomes of the major bacterial taxa associated to the samples. Together, our results demonstrate that, although seed microbiome varied across plant species, nutrient availability during germination elicits changes of the composition of microbial communities by selecting microbial groups with specific copiotrophic functional traits. The data presented here represents the first attempts to empirically assess changes in the microbial community during plant emergence and moves us towards a more holistic understanding of the plant microbiome.

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